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Effect of periportal-and centrilobular-equivalent oxygen tension on liver specific functions in long-term rat hepatocyte cultures

Authors: 
P Maier, B Saad, HP Schawalder
Journal Name: 
Toxicology in vitro
Volume: 
8
Issue: 
3
Pages From: 
423
To: 
435
Date: 
الخميس, يونيو 30, 1994
Abstract: 
The influence of periportal-and centrilobular-equivalent oxygen tensions on cellular functions and xenobiotic metabolism was investigated in rat hepatocytes cultured under 20%(air/CO 2), 13% or 4% O 2 for up to 9 days on teflon membrane dishes, coated with crude membrane fractions and collagen. Protein content, total cytochrome P-450 content, 7- ethoxy-resorufin-O-deethylase (EROD) and the profile of hydroxytestosterone metabolites were not significantly affected by the oxygen tension, whereas intracellular lactate ...