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Oxygen tension, insulin, and glucagon affect the preservation and induction of cytochrome P450 isoforms in cultured rat hepatocytes

Authors: 
Bashar Saad, Helmut Thomas, Hanspeter Schawalder, Felix Waechter, Peter Maier
Journal Name: 
Toxicology and applied pharmacology
Volume: 
126
Issue: 
2
Pages From: 
372
To: 
379
Date: 
الأربعاء, يونيو 1, 1994
Abstract: 
The role of oxygen tension, insulin, and glucagon on the preservation and induction of cytochrome P450 isoenzyme activities and contents was investigated in rat hepatocytes cultured for 4 days on crude liver membrane fractions at 4 or 13% O2. At 13% O2, three out of six immunochemically analyzed P450 isoenzymes were significantly higher than in 4% O2. Exposure to phenobarbital (PB) from Days 1 to 4 dose dependently increased the protein content and decreased the albumin secretion in 13% O2 cultures only. The ...